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anti rabbit igg secondary antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti rabbit igg secondary antibody
    Anti Rabbit Igg Secondary Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+rabbit+secondary+antibody/pmc13022602-150-5-9
    Average 86 stars, based on 1 article reviews
    anti rabbit igg secondary antibody - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Reprogramming of lipid metabolism to inhibit T cell senescence and enhance tumor immunotherapy
    Article Snippet: .. The treated CD4+ T cells were then incubated with BODIPY 493/503 and the Alexa Fluor 594-conjugated anti-rabbit secondary antibody (Cell Signaling Technology), and were further counterstained with 40, 6-diamidino-2-phenylindole (DAPI; Invitrogen). ..

    Article Title: Oncolytic virus compositions including IL-15 complex and methods for the treatment of cancer
    Article Snippet: Samples were loaded for sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and separated proteins were transferred onto the PVDF membranes (BIO-RAD #100-26933). .. After blocking the membrane with 5% bovine serum albumin (BSA, Sigma-Aldrich #A3294-10G), the primary anti-HA-tag antibody (Cell Signaling Technology (CST), clone: C29F4, #3724) was added for an overnight incubation at 4° C., followed by an incubation with anti-rabbit secondary antibody (CST #7074) for one hour at room temperature. ..

    Article Title: Aging Impairs Intramuscular Collagen Remodeling Responses to Repeated Passive Stretching in Skeletal Muscle.
    Article Snippet: The sections were incubated overnight at 4 ◦C with rabbit polyclonal anti-collagen VI antibody (ab6588; Abcam, Cambridge, MA, USA) diluted 1:500 in PBS containing 0.3% Triton X-100. .. Next, the sections were incubated at 25 ◦C for 1 h with an Alexa Fluor 555-labeled anti-rabbit secondary antibody (4413S, Cell Signaling Technology, Danvers, MA, USA) diluted 1:1000 in PBS. ..

    Article Title: Evolution of a distinct chromatin regulatory landscape in brown algae.
    Article Snippet: These included rabbit anti-H3 (Histone H3 (D2B12) XP Rabbit mAb (ChIP Formulated), Cell Signaling Technology, 4620S), anti-H3K4me3 (Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb, CST, 9751S), anti-H3K9ac (Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb, CST, 9649S), anti-H3K79me2 (Di-Methyl-Histone H3 (Lys79) (D15E8) XP Rabbit mAb, CST, 5427S, and anti-H4K20me3 (Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb, CST, 5737S) at 1:1,000 dilution, and anti-H3K36me3 (AbcamTri-Methyl-Histone H3 (Lys36) (D5A7) XP Rabbit mAb, Abcam, 4909S) at 1 μg ml−1. .. Membranes were incubated with HRP-conjugated anti-rabbit secondary antibody (1:2,000, CST, 7074S). .. After further washes, membranes were developed using a 1:1 mix of Trans-Blot Turbo Transfer System (BioRad, 1704150).

    Article Title: HNRNPH1 drives glioblastoma progression by regulating the splicing of cell cycle genes.
    Article Snippet: Tissue sections were incubated with primary anti-HNRNPH1 antibody (A300-511A, Bethyl Laboratories, Montgomery, TX, USA) in TBST containing 2% normal goat serum for 1 hour at RT. .. After washing, sections were incubated with horseradish peroxidase-conjugated anti-rabbit secondary antibody (Cell Signaling, Danvers, MA, USA) in TBST for 30 minutes at RT. .. Sections were stained using DAB substrate (Thermo Fisher Scientific) and counterstained in hematoxylin.

    Article Title: H3K4 methylation of CALB2 facilitates immune evasion and chemoradiotherapy resistance in cholangiocarcinoma through KRT7-mediated PD-L1 upregulation.
    Article Snippet: This study elucidates how H3K4 methylation-driven upregulation of Calbindin 2 (CALB2) promotes immune evasion and chemoradioresistance in cholangiocarcinoma (CCA).. Integrated bioinformatics analyses identified CALB2 as a hub gene linked to an immunosuppressive microenvironment.. In vitro, CALB2 knockdown suppressed tumor cell proliferation, migration, invasion, and calcium signaling—evidenced by reduced phosphorylation of CaMKII, PKC, and NF-κB—while increasing apoptosis.

    Article Title: Pirfenidone Reduces Intracochlear Fibrosis Caused by Cochlear Implantation in a Guinea Pig Model
    Article Snippet: .. Membranes were washed in TBS-T and incubated with HRPconjugated anti-rabbit secondary antibody (Cell Signalling Technology, Danvers, MA, USA) for 1 h at room temperature; blots were washed in TBS-T again and incubated in enhanced ECL (Millipore/Sigma Aldrich, St Louis, MO, USA) for 5 min. Membranes were visualised using the iBright imaging system (Invitrogen/Thermo Fisher Scientific) and analysed using FIJI imaging software (Image J2, version 2.14.011.54f) [68]. ..

    Blocking Assay:

    Article Title: Oncolytic virus compositions including IL-15 complex and methods for the treatment of cancer
    Article Snippet: Samples were loaded for sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and separated proteins were transferred onto the PVDF membranes (BIO-RAD #100-26933). .. After blocking the membrane with 5% bovine serum albumin (BSA, Sigma-Aldrich #A3294-10G), the primary anti-HA-tag antibody (Cell Signaling Technology (CST), clone: C29F4, #3724) was added for an overnight incubation at 4° C., followed by an incubation with anti-rabbit secondary antibody (CST #7074) for one hour at room temperature. ..

    Membrane:

    Article Title: Oncolytic virus compositions including IL-15 complex and methods for the treatment of cancer
    Article Snippet: Samples were loaded for sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and separated proteins were transferred onto the PVDF membranes (BIO-RAD #100-26933). .. After blocking the membrane with 5% bovine serum albumin (BSA, Sigma-Aldrich #A3294-10G), the primary anti-HA-tag antibody (Cell Signaling Technology (CST), clone: C29F4, #3724) was added for an overnight incubation at 4° C., followed by an incubation with anti-rabbit secondary antibody (CST #7074) for one hour at room temperature. ..

    Imaging:

    Article Title: Pirfenidone Reduces Intracochlear Fibrosis Caused by Cochlear Implantation in a Guinea Pig Model
    Article Snippet: .. Membranes were washed in TBS-T and incubated with HRPconjugated anti-rabbit secondary antibody (Cell Signalling Technology, Danvers, MA, USA) for 1 h at room temperature; blots were washed in TBS-T again and incubated in enhanced ECL (Millipore/Sigma Aldrich, St Louis, MO, USA) for 5 min. Membranes were visualised using the iBright imaging system (Invitrogen/Thermo Fisher Scientific) and analysed using FIJI imaging software (Image J2, version 2.14.011.54f) [68]. ..

    Software:

    Article Title: Pirfenidone Reduces Intracochlear Fibrosis Caused by Cochlear Implantation in a Guinea Pig Model
    Article Snippet: .. Membranes were washed in TBS-T and incubated with HRPconjugated anti-rabbit secondary antibody (Cell Signalling Technology, Danvers, MA, USA) for 1 h at room temperature; blots were washed in TBS-T again and incubated in enhanced ECL (Millipore/Sigma Aldrich, St Louis, MO, USA) for 5 min. Membranes were visualised using the iBright imaging system (Invitrogen/Thermo Fisher Scientific) and analysed using FIJI imaging software (Image J2, version 2.14.011.54f) [68]. ..



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